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DNA甲基轉(zhuǎn)移酶3B（DNMT3B）分析試劑盒
DNA甲基轉(zhuǎn)移酶3B（DNMT3B）分析試劑盒設(shè)計(jì)用于測(cè)量組織或細(xì)胞的Dnmt3B總量。在用該試劑盒進(jìn)行檢測(cè)時(shí)，獨(dú)特的Dnmt親和底物被穩(wěn)定地涂在條形孔上。將樣品加入孔中，樣品中含有的Dnmt3B與底物結(jié)合。結(jié)合的Dnmt3B可以用特異性Dnmt3B抗體識(shí)別，并通過(guò)類似ELISA的反應(yīng)進(jìn)行比色定量。Dnmt3B的數(shù)量與顯色的強(qiáng)度成正比。
DNA甲基轉(zhuǎn)移酶3B（DNMT3B）分析試劑盒是一個(gè)方便的工具包，允許實(shí)驗(yàn)者以極快的速度和一致性測(cè)量Dnmt3B的數(shù)量；比目前所有其他方法更優(yōu)越和安全。該試劑盒是即用型的，提供了成功進(jìn)行Dnmt3B檢測(cè)實(shí)驗(yàn)所需的所有基本成分。DNA甲基轉(zhuǎn)移酶3B（DNMT3B）分析試劑盒適用于定量測(cè)量人類和小鼠的新鮮組織和培養(yǎng)細(xì)胞中的Dnmt數(shù)量。
合作開(kāi)發(fā)的DNA甲基轉(zhuǎn)移酶3B（DNMT3B）分析試劑盒。具有如下的優(yōu)勢(shì)和特性：
- 超快的程序，可在3小時(shí)內(nèi)完成。
- 創(chuàng)新的比色法，無(wú)需電泳就能定量測(cè)量Dnmt3B的含量。
- 條形微孔板格式，使檢測(cè)靈活：手工或高通量分析。
- 簡(jiǎn)單、可靠、一致的檢測(cè)條件。
產(chǎn)品組分
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組分內(nèi)容 |
型號(hào)：A-P-3013-48(48次) |
型號(hào)：A-P-3013-96(96次) |
儲(chǔ)存溫度 |
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DB1 |
10x Wash Buffer |
12 ml | 25 ml | 4°C |
| DB2 | Assay Buffer | 5 ml | 10 ml | 4°C |
| DB3 |
DNMT3B Standard,20ug/ml* |
16 ul | 30 ul | 室溫 |
| DB4 | Capature Antibody,100ug/ml* | 8 ul | 16 ul | 4°C |
| DB5 |
Detection Antibody,200ug/ml* |
10 ul | 20 ul | 室溫 |
| DB6 | Developing Solution* | 6 ml | 12 ml | 4°C |
| DB7 |
Stop Solution |
6 ml | 11 ml | 室溫 |
| BB | Blocking Buffer | 10 ml | 20 ml | 4°C |
|
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8-Well Assay Strips(With Frame) |
6 | 12 | 4°C |
|
操作手冊(cè) |
|
1 |
1 |
室溫 |
文件資源
| 文件下載 | 文件內(nèi)容 | 資源說(shuō)明 |
 |
A-P-3013-DNA甲基轉(zhuǎn)移酶3B（DNMT3B）分析試劑盒 | 操作手冊(cè) |
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A-P-3013-DNA甲基轉(zhuǎn)移酶3B（DNMT3B）分析試劑盒相關(guān)產(chǎn)品 | 宣傳單頁(yè) |
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注意事項(xiàng)
| 保存建議 | 推薦藍(lán)冰或冰袋運(yùn)輸。當(dāng)您收到產(chǎn)品后，按照說(shuō)明書(shū)建議保存各組分。 |
| 警告 | 本品僅供科研使用，請(qǐng)勿用于臨床與診斷。 |
FAQ
Siqueira IR et. al. (September 2020). Correlation Between Inflammatory and Epigenetic Marks With Aerobic Performance in 10-km Runners. Res Q Exerc Sport. :1-10.
Mccoy CR et. al. (January 2019). Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High Versus Low Anxiety. J Neurosci.
Coco M et. al. (November 2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise. Medicine (Baltimore). 96(44):e8325.
Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.
Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.
Horsburgh S et. al. (December 2015). Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiol Rep. 3(12)
Zhao J et. al. (January 2013). Genome-wide identification of Epstein-Barr virus-driven promoter methylation profiles of human genes in gastric cancer cells. Cancer. 119(2):304-12.
Jin Y et. al. (June 2011). Homocysteine levels impact directly on epigenetic reprogramming in astrocytes. Neurochem Int. 58(7):833-8.
Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.
Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.
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