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m6A兔源多克隆抗體
【產(chǎn)品背景】
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
【應(yīng)用類型】
WB: 1 : 1000 up to 1 : 10000 (AP staining) suitable for Dot Blot gallery
IP: yes
ICC: 1 : 200
IHC: not tested yet
IHC-P/FFPE: 1 : 100
ELISA: yes suitable for sandwich-ELISA gallery
【免疫原】
N6-methyladenosine fused to BSA.
【反應(yīng)性】
Reacts with: human, rat, mouse, eukaryotes, prokaryotes.
Other species not tested yet.
【特異性】
Specific for N6-methyladenosine (m6A) with some cross-reactivity to m6Am.
【說明書】
產(chǎn)品組分
內(nèi)容 |
型號(hào) |
規(guī)格 | 價(jià)格 |
儲(chǔ)存溫度 |
m6A兔源多克隆抗體 | 202003 | 50ug | 0.00 | -20°C |
操作手冊(cè) |
1 | 1 |
常溫 |
操作手冊(cè)
文件下載 | 文件內(nèi)容 | 資源說明 |
202003-m6A兔源多克隆抗體 | 操作手冊(cè) | |
202003-m6A兔源多克隆抗體相關(guān)說明 | 宣傳單頁 |
注意事項(xiàng)
保存建議 | 推薦藍(lán)冰或冰袋運(yùn)輸。當(dāng)您收到產(chǎn)品后,按照說明書建議保存各組分。 |
FAQ
條件。
HIV-1 envelope proteins up-regulate N6-methyladenosine levels of cellular RNA independently of viral replication.
Tirumuru N, Wu L
The Journal of biological chemistry (2019) : . 202 003 WB, DOTBLOT; tested species: human
Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides.
Mishima E, Abe T
Methods in molecular biology (Clifton, N.J.) (2019) 1870: 179-187. 202 003 WB
Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides.
Mishima E, Jinno D, Akiyama Y, Itoh K, Nankumo S, Shima H, Kikuchi K, Takeuchi Y, Elkordy A, Suzuki T, Niizuma K, et al.
PloS one (2015) 1011: e0143756. 202 003 WB, DOTBLOT
N6-methyladenine DNA Modification in Glioblastoma.
Xie Q, Wu TP, Gimple RC, Li Z, Prager BC, Wu Q, Yu Y, Wang P, Wang Y, Gorkin DU, Zhang C, et al.
Cell (2018) : . 202 003 DOTBLOT, IP, ICC, IHC-P; tested species: human
Melatonin restores the pluripotency of long-term-cultured embryonic stem cells through melatonin receptor-dependent m6A RNA regulation.
Yang L, Liu X, Song L, Su G, Di A, Bai C, Wei Z, Li G
Journal of pineal research (2020) 692: e12669. 202 003 DOTBLOT, IP; tested species: mouse
Leukemogenic Chromatin Alterations Promote AML Leukemia Stem Cells via a KDM4C-ALKBH5-AXL Signaling Axis.
Wang J, Li Y, Wang P, Han G, Zhang T, Chang J, Yin R, Shan Y, Wen J, Xie X, Feng M, et al.
Cell stem cell (2020) : . 202 003 DOTBLOT; tested species: human
YTHDF2 promotes spermagonial adhesion through modulating MMPs decay via m6A/mRNA pathway.
Huang T, Liu Z, Zheng Y, Feng T, Gao Q, Zeng W
Cell death & disease (2020) 111: 37. 202 003 DOTBLOT, IP; tested species: mouse
Mammalian ALKBH1 serves as an N6-mA demethylase of unpairing DNA.
Zhang M, Yang S, Nelakanti R, Zhao W, Liu G, Li Z, Liu X, Wu T, Xiao A, Li H
Cell research (2020) : . 202 003 DOTBLOT
FTO Demethylates Cyclin D1 mRNA and Controls Cell-Cycle Progression.
Hirayama M, Wei FY, Chujo T, Oki S, Yakita M, Kobayashi D, Araki N, Takahashi N, Yoshida R, Nakayama H, Tomizawa K, et al.
Cell reports (2020) 311: 107464. 202 003 DOTBLOT, IP; tested species: mouse
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