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組蛋白H3K27甲基轉(zhuǎn)移酶活性/抑制分析試劑盒（比色法）
組蛋白H3K27甲基轉(zhuǎn)移酶活性/抑制分析試劑盒（比色法）是一套方便的工具，允許實(shí)驗(yàn)者測(cè)量特異性靶向組蛋白H3賴氨酸27 (H3- K27)的單個(gè)組蛋白甲基轉(zhuǎn)移酶的活性或抑制。 該試劑盒可隨時(shí)使用，并提供進(jìn)行成功的HMT活性/抑制實(shí)驗(yàn)所需的所有必要成分，而不需要放射性或任何特殊設(shè)備。
組蛋白H3K27甲基轉(zhuǎn)移酶活性/抑制分析試劑盒（比色法）是專為測(cè)量組蛋白H3在賴氨酸27的HMT而設(shè)計(jì)的。 在使用該試劑盒的試驗(yàn)中，組蛋白底物被穩(wěn)定地捕獲在條帶孔上。 HMT酶將一個(gè)甲基從Adomet轉(zhuǎn)移到組蛋白H3底物，使底物在賴氨酸9處甲基化。 甲基化組蛋白H3-K27可被高親和力抗體識(shí)別。 甲基化H3-K27的比例或數(shù)量與酶活性成正比，可以通過HRP共軛的二級(jí)抗體顯色系統(tǒng)進(jìn)行定量。 然后根據(jù)HMT轉(zhuǎn)化H3-K27的甲基化量計(jì)算HMT活性。
組蛋白H3K27甲基轉(zhuǎn)移酶活性/抑制分析試劑盒（比色法），具有如下優(yōu)勢(shì)和特點(diǎn)：
- 非?？焖俚某绦颍梢栽?小時(shí)內(nèi)完成 。
- 安全、創(chuàng)新的比色分析，無需放射性、提取和色譜。
- H3-K27組蛋白甲基轉(zhuǎn)移酶活性/抑制的特異性測(cè)定。
- 微孔板條狀格式使分析靈活:手工或高通量分析。
- 極其簡(jiǎn)單、可靠和一致的分析條件。
產(chǎn)品組分
|
組分內(nèi)容 |
型號(hào):A-P-3005-48（48次） |
型號(hào):A-P-3005-96（96次） |
儲(chǔ)存溫度 |
| HE1(10X Wash Buffer) | 11ml | 22ml | 4°C |
| HE2(Histone Assay Buffer) | 1.5ml | 3ml | 4°C |
| HE3(Adomet)* | 25ul | 50ul | -20°C |
| HE4(Biotinylated Substrate,25ug/ml)* | 100ul | 200ul | -20°C |
| HE5(HMT Standard,10ug/ml)* | 10 ul |
20 ul |
-20°C |
| HE6(Capture Antibody,100ug/ml)* | 25 ul |
50 ul |
4°C |
| HE7（Detetion Antibody,200ug/ml）* | 10ul | 20ul | -20°C |
| HE8（Developer Solution） | 6ml | 12ml | 4°C |
| HE9（Stop Solution）* | 3ml | 6ml | 室溫 |
| Control Enzyme（300ug/ml）* | 5ul | 10ul | -20°C |
| 8-Well Assay Strips(With Frame) | 6 | 12 | 4°C |
|
說明書 |
1份 | 1份 |
室溫 |
文件資源
| 文件下載 | 文件內(nèi)容 | 資源說明 |
 |
A-P-3005-組蛋白H3K27甲基轉(zhuǎn)移酶活性/抑制分析試劑盒（比色法） | 操作手冊(cè) |
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A-P-3005-組蛋白H3K27甲基轉(zhuǎn)移酶活性/抑制分析試劑盒（比色法）相關(guān)說明 | 宣傳單頁 |
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FAQ
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